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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential job in selling cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already explained. Two added splice variants were documented but in depth nucleotide sequence information and facts is just not but out there.
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Blocking peptides are peptides that bind specifically to the goal antibody and block antibody binding. These peptide usually has the epitope acknowledged because of the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to guarantee the absolute best results are...
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This guideline outlines additional possible optimization of cellular and immunoassay parameters to be sure the best possible final results are...
Antibodies certain to the blocking peptide no more bind into the epitope over the concentrate on protein. By evaluating the staining within the blocked antibody compared to the antibody alone, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and capabilities being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates within the G1-S section boundary which is degraded as cells progress by S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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